BIOCHEMICAL STUDIES ON RICE BLAST DISEASE. PART 10. BIOCHEMICAL CLASSIFICATION OF PIRICULARIA ORYZAE CAVARA (5)
ARMY BIOLOGICAL LABS FREDERICK MD
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Employing 47 strains of piricularia oryzae, a test was made of the utilization of organic salts involved in the T.C.A. cycle, the utilization of cellulose and the differences in pectinase, as well as the amount of biotin produced by strains 1 and 2. Strains 1 and 2, which do not require biotin, were studied by cultivating them in a biotin-free culture medium to see if they are capable of producing biotin. As a result of testing the amount of biotin by bioassay of the acid hydrolizate of rinsed mycelia and of culture filtrate, it was found that these strains do not produce biotin. These strains are believed to have a different type of sugar metabolism than the other strains. The utilization of the culture medium containing succinic acid, fumaric acid, and citric acid, and a medium containing the above acids plus 1100 M of malonic acid was tested. The results lead us to believe that nine strains follow a course different from the T.C.A. cycle. Although a correlation was observed between utilization and pathogenicity in the tests on the utilization of cellulose, this correlation cannot provide a clue to classification. No marked differences for pectinase were detected. No clue was found to classification from the above results on pectinase and hydrolizability of cellulose.