ON THE QUESTION OF THE REDUCTION OF 2,3,5-TRIPHENYL TETRAZOLIUM CHLORIDE BY MICROORGANISMS BELONGING TO DIFFERENT FAMILIES
ARMY BIOLOGICAL LABS FREDERICK MD
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After a preliminary discussion of the problem of the behavior of a microorganism with respect to its environment with reference to its dissimilatory-metabolic processes, the biological reduction processes are discussed along with their measurement by transferring tetrazolium salts into formazan. The reaction potentialities were studied by using 2, 3, 5-triphenyl tetrazolium chloride TTC on 95 different strains that belong to 12 families of microorganisms. The TTC test, performed without the addition of a substrate, yielded clear differences in the reduction ability of the various strains, which were found to depend on the pH value. It was determined that TTC reduction is, indeed, a reaction that is possible in all strains but one that is very dependent on the conditions. A sure differentiation into parasitic and saprophytic strains occurs only with Mycobacteria and Candidae. From these research results, it seems likely that a differentiation of the strains of the other families will also be possible by means of appropriate experiments using specific substrates.