ADDITIONAL STUDIES OF MICROTOMIC SECTIONS OF CANARY POX VIRUS
Rept. no. 4
FORT DETRICK FREDERICK MD
Pagination or Media Count:
It was demonstrated in an earlier report on the electron microscopy of canary pox virus that the sites of inception of elementary body EB development are scattered throughout the cytoplasm. The form and internal structure of this EB were described and visualized in two model sections in perspective. Thus two areas in the plasma of infected histiocytes were relevant for future studies, depending on the point of attack either the Bollinger bodies or virus vesicles, if the ultrastructure of mature EB was to be investigated, or the developmental sites of early forms scattered throughout the cytoplasm, if information was desired on chronological factors in the genesis of the first EB membranes or the finely granular densities enclosed by them. The present study employed virus-infected histiocytes embedded in the polyester vestopal W recommended by Kellenberger. This as well as other methacrylate- embedded preparations were suspended in agar after fixation and subjected to further treatment.