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STUDY OF THE VARIABILITY OF THE TICK-BORNE VIRUS OF ENCEPHALITIS. REPORT 1. LONG CULTIVATION OF VIRUSES OF THE TICK-BORNE ENCEPHALITIS GROUP IN RENAL- CELL CULTURE OF PIG EMBRYO AND IN CHICK EMBRYOS
ARMY BIOLOGICAL LABS FREDERICK MD
Pagination or Media Count:
As a result of the prolonged passaging in a culture of pig-embryo renal cells at 33C 70 passages and at 40C 80 passages, as well as in developing chick embroys 120 passages of the viruses of the tick-borne encephalitis group strains Ix-10, Pan, and I-40, the neuropathogenic properties of these strains for white mice have not been changed. As a result of the adaptation, certain strains have acquired the aptitude to destroy SOTs cells and fibroblasts of chick embryos. Pan and I-40 strains, both the initial and the variants obtained, have possessed the property to form plaques in culture of pig-embryo renal cells. Variants of the viruses, obtained at growing in cultures of pig-embryo renal cells at 40C Pan and I-40, at 33C, and in thick embryos I-40, have acquired the capacity to form plaques in cultures of SOTs cells.
APPROVED FOR PUBLIC RELEASE