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A STUDY OF 'IMMEDIATE' SENSITIZATION BY ADSORPTION OF ANTIGENS AND ANTIBODIES IN VITRO
Interim technical rept. Sep 1967-Feb 1968
STANFORD UNIV CA SCHOOL OF MEDICINE
Pagination or Media Count:
The interaction of several sea urchin toxin preparations with certain substrates in mammalian serum produces dialyzable, heat-stable substances which cause contractions of the guinea pigs ileum and rats uterus. Evidence is presented to show that the toxin acts kinetically as an enzyme and that one of the substrates is electrophoretically pure human alpha 2-macroglobulin. Crude toxin preparations were also shown to be kininolytic with respect to the dialyzable reaction-product as well as to synthetic bradykinin. Rabbit antibodies to formalinized toxoids quantitatively precipitated active toxin, fixed complement, and protected mice against lethal intravenous doses in the usual quantitative protection and neutralization tests. Immunoelectrophoretic analysis brought out the existence of two distinct antigenic determinants, and additional serological tests showed that antibodies directed against the pedicellarial toxins of T. gratilla cross-reacted with test proteins of S. purpuratus.
APPROVED FOR PUBLIC RELEASE