A RAPID TURBIDIMETRIC PROCEDURE FOR COMPLEMENT ESTIMATION
FORT DETRICK FREDERICK MD
Pagination or Media Count:
Complement C-mediated hemolysis of sheep erythrocytes followed a first-order kinetics reaction when measured turbidimetrically. A linear standard curve can be constructed from the slopes of the first-order plots obtained with samples of known C concentration. The standard curve can then be used for the estimation of C concentration in unknown sera. This direct kinetic procedure is rapid and accurate and employs a minimum of equipment and reagents.