PATHOLOGICAL CHANGES IN RABBITS INJECTED WITH PASTEURELLA TULARENSIS KILLED BY IONIZING RADIATION
FORT DETRICK FREDERICK MD
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Suspensions of Pasteurella tularensis killed by exposure to ionizing radiation were toxic for rabbits. A dose of 3 ml or more of a suspension containing 2 x 10 to the 11th power to 3 x 10 to the 11th power organisms per ml was lethal within 24 hours of a single intravenous injection. The major pathologic changes were extensive hemorrhagic necrosis of the spleen, focal coagulation necrosis of the liver, pneumonitis, and glomerular capillary occlusion by fibrin thrombi. Similar lesions were produced when two smaller doses of the suspension were given intravenously 24 hours apart. The localized Shwartzman reaction was produced by an intradermal injection followed in 24 hours by an intravenous injection. Rabbits were protected against the lethal action of the suspension and against development of glomerular thrombosis by prior administration of either a single dose or of multiple doses of 25 mg of cortisone.
- Medicine and Medical Research