EFFECT OF PHYSICAL FACTORS ON RED CELLS SUBJECT TO FREEZING AND THAWING.
Progress rept. no. 60, 1 Oct 65-31 Oct 66,
JOHN S SHARPE RESEARCH FOUNDATION BRYN MAWR PA
Pagination or Media Count:
Development of the technique of ATP determination, biological variations and application to the study of red cells stored in the liquid and frozen state were carried out, and the results are reported in Progress Report No. 59, dated September 15, 1966. Reference to this report is made in Progress Report No. 60, whenever necessary, so that interested investigators may obtain details of technique and results reported. Because of similarity of effect whether the addition was made before or after freezing or thawing, in the present series of experiments, additions were made invariable before freezing and thawing. Flame photometry and atomic absorption technique applied to the measurement of K and Na in blood subjected to freezing and thawing gave comparable results. Significant loss of K amounting to approximately 50 of the amount present in red cells of fresh blood was found to occur upon freezing and thawing. Incubation of the thawed red cells was not followed by reentry of K into red cells. There is apparently no relationship of red cell content of K and ATP of frozen red cells and their recovery in vitro and posttransfusion survival. Blood frozen after the addition of adenine and inosine showed, upon incubation, increase in the ATP content, however, no improvement of the posttransfusion survival was noted of red cells thus treated, contrarily to what happens to red cells stored in the conventional manner in the liquid state. The increase of ATP of frozen red cells, incubated with adenine and isosine occurs only in the presence of buffered electrolytes. The addition of PO4 is essential for the regeneration of ATP the addition of Mg appears to have no effect.
- Anatomy and Physiology