ALTERATION OF THE PENETRABILITY OF SKIN.
Final rept. Jul-Dec 66,
LEVER BROS CO EDGEWATER NJ RESEARCH AND DEVELOPMENT DIV
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Oxidized lipid-protein complexes were investigated as a means of increasing the barrier properties of skin. Membranes were formed from these complexes, and the optimum lipid to protein ratios was from 1 to 2 parts by weight protein to 2.5 parts by volume lipid. Incorporation of cholesterol in these membranes lowered the water permeability of the membrane to values found for natural stratum corneum. The membranes showed greater resistance to the destructive action of organic solvents than natural stratum corneum. Differential thermal analysis of the membranes indicated that most of the lipids were complexed with protein. Neonatal rat skin, which was damaged by solvent extraction, was treated with various combinations of lipid-protein complexes. Among various combinations tested, mixtures of linoleic acid, corneum cells, and cholesterol were found most effective in restoring barrier properties to the solvent damaged skin. Evaluation of different materials as possible wound-healing agents were undertaken in a series of studies in guinea pigs. In situ membranes consisting of albumin, safflower oil and cholesterol and a suspension of epidermal cells accelerated epithelialization of partial thickness wounds. Corneum cell membranes and dissociated corneum cells were found to accelerate epithelialization of full thickness wounds. Author
- Anatomy and Physiology
- Coatings, Colorants and Finishes