EFFECT OF UV-IRRADIATED HELPER PHAGE ON TRANSFORMATION AND TRANSFECTION OF ESCHERICHIA COLI BY DNA FROM A TRANSDUCING PHAGE
FORT DETRICK FREDERICK MD
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When moderately irradiated helper phage lambda i434 was added to Escherichia coli W1485 A23 try A- cells and DNA from a non-defective, temperate transducing hybrid phage carrying bacterial try A and B genes i lambda a h theta 80 AB, it resulted in an increase in try transformants and hybrid phage transfectants. The increase may result from prevention of lysis of potential transformants and transfectants because of irradiation damage in the helper phage. The addition of severely irradiated lambda i434 resulted in a decrease in the number of transfectants but did not reduce the number of transformants. Two possibilities are considered the decrease in transfectants results from insertion of ultraviolet-light damage in the helper phage DNA into the genome of the hybrid phage, preventing replication of the hybrid phage and the registry of the try genes carried by the hybrid phage with the bacterial chromosome, resulting in try transformants, is not affected by insertion of ultraviolet light damage from the helper phage.