HISTOCHEMICAL BETA-GLUCURONIDASE DISTRIBUTION IN MAMMALIAN TISSUE AS DETECTED BY 5-BROMO-4-CHLOROINDOLE-3-YL-BETA-D-GLUCOPYRURONISIDE
FORT DETRICK FREDERICK MD
Pagination or Media Count:
A new substrate, 5-bromo-4-chloroindol-3-yl-beta-D-glucopyruroniside, has been used to demonstrate beta-D-glucuronidase in rat tissues. The reaction requires neither ferri-ferrocyanide nor a final coupling step. The optimal pH range is 4.0 to 5.4. The reaction is specific and can be readily distinguished from that of other glycosidases by inhibition with lactone. The wide tissue distribution observed is, in general, similar to that described in previous studies of tissue homogenates and histochemical studies. Because of the advantages of indoxyl substrates the method is applicable to the study of beta-D-glucuronidase in pathological conditions.