Accession Number:

AD0810492

Title:

EFFECT OF INPUT MULTIPLICITY AND TISSUE CELL CONCENTRATION UPON THE GROWTH OF RIFT VALLEY FEVER VIRUS

Descriptive Note:

Corporate Author:

FORT DETRICK FREDERICK MD

Personal Author(s):

• Orlando, Michael D.
• DeLauter, Richard D.
• Riley, Jean M.

Report Date:

1967-03-01

Pagination or Media Count:

13.0

Abstract:

The growth of Rift Valley fever RVF virus in L Earle cells obtained from two sources and the effect of input multiplicity and tissue cell concentration upon the growth of the virus are described. The titers obtained in the suspension cultures with the cells from two laboratories were significantly different. With both monolayer culture system and suspension culture system, an input multiplicity of 2.5 resulted in the greatest proliferation of virus. Optimal viral yield was obtained in suspension cultures with 40,000,000 tissue cells per 100 ml of suspension.

Descriptors:

• *TISSUE CULTURE CELLS
• *RIFT VALLEY FEVER VIRUS
• GROWTH(PHYSIOLOGY)
• CULTURE MEDIA
• TISSUE CULTURE

Subject Categories:

• Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE