Accession Number:

AD0810287

Title:

A PRECISE METHOD FOR REPLICATING SUSPENSION CULTURES OF MAMMALIAN CELLS,

Descriptive Note:

Corporate Author:

FORT DETRICK FREDERICK MD

Personal Author(s):

• Weirether, Francis J.

Report Date:

1967-02-01

Pagination or Media Count:

12.0

Abstract:

A simple, readily assembled shaker culture system for the cultivation of mammalian cells is described and illustrated. It uses no specialized glassware and equipment and consists of an Erlenmeyer flask fitted with a breather-sampling assembly. This unit was employed in quantitating the effects of several variables including medium ingredients, serial transfers, and freezing and storage on two variants of the L cell line. The method is sensitive and reproducible and allows for growth of cells in suspension for extended periods of time. Large numbers of cells can be mass-produced. Many replicates can be run simultaneously to yield data for statistical analysis. Author

Descriptors:

• (*TISSUE CULTURE CELLS
• TEST METHODS)
• CULTURE MEDIA
• GROWTH(PHYSIOLOGY)
• LABORATORY EQUIPMENT
• STATISTICAL ANALYSIS
• MAMMALS
• REPRODUCTION

Subject Categories:

• Anatomy and Physiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE