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A PRECISE METHOD FOR REPLICATING SUSPENSION CULTURES OF MAMMALIAN CELLS,
FORT DETRICK FREDERICK MD
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A simple, readily assembled shaker culture system for the cultivation of mammalian cells is described and illustrated. It uses no specialized glassware and equipment and consists of an Erlenmeyer flask fitted with a breather-sampling assembly. This unit was employed in quantitating the effects of several variables including medium ingredients, serial transfers, and freezing and storage on two variants of the L cell line. The method is sensitive and reproducible and allows for growth of cells in suspension for extended periods of time. Large numbers of cells can be mass-produced. Many replicates can be run simultaneously to yield data for statistical analysis. Author
APPROVED FOR PUBLIC RELEASE