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CHROMOSOMAL MAPPING IN STRAINS OF STAPHYLOCOCCUS AUREUS,
FORT DETRICK FREDERICK MD
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The nitrosoguanidine method of mapping the chromosome of Staphylococcus aureus is described. The method employs phenylethyl alcohol inhibition of chromosomal replication followed by periodic exposure to nitrosoguanidine after release from the phenylethyl alcohol block. Chromosomal replication requires approximately 60 minutes under the standard conditions employed. A genomic map of a phage type 8081 strain containing the locations of 10 genes has been obtained. Comparison of a number of isolates of S. aureus from clinical materials for the location of resistance loci to chloramphenicol, novobiocin, erythromycin, nitrofurazone, and acriflavin has shown clear transpositions of some loci. No evidence has been obtained to indicate either a possible circular chromosome or differences in polarity of chromosomal replication among the strains examined. It appears that the order of the resistance genes examined may vary considerably among the strains so far evaluated. Author
APPROVED FOR PUBLIC RELEASE