Accession Number:

AD0806775

Title:

STUDIES ON THE COCCIDIUM, EIMERIA TENELLA,

Descriptive Note:

Corporate Author:

FORT DETRICK FREDERICK MD

Report Date:

1966-12-01

Pagination or Media Count:

24.0

Abstract:

Essentially pure sporulated Eimeria tenella oocyst suspensions, suitable for experimental purposes, were obtained by shaking at 30 C homogenized infected chicken ceca in 2.5 potassium dichromate, digesting extraneous material with 3 KOH at 40 C in the presence of fecal debris, with an additional incubation in 70 H2SO4, and further purifying by selective recovery from the aqueous phase of a chloroform-water suspension. A quantitative assay of infecting dose in chicks could be made by harvesting ceca after 7 days and counting total oocysts oocyst viability could be determined by per cent in vitro excystation of sporocysts. Oocysts failed to survive freezing in liquid nitrogen or drying, but survived for short periods at -5 to -30 C. Ammonium hydroxide, aqueous iodine, and ethylene oxide were the only disinfectants found effective against E. tenella oocysts. Cross-infection studies with other animal species were uniformly unsuccessful. Author

Subject Categories:

  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE