Accession Number:

AD0806660

Title:

MUTATIONS INFLUENCING THE ASSIMILATION OF NITROGEN BY PASTEURELLA PESTIS,

Descriptive Note:

Corporate Author:

FORT DETRICK FREDERICK MD

Personal Author(s):

Report Date:

1967-01-01

Pagination or Media Count:

28.0

Abstract:

Cells of Pasteurella pseudotuberculosis, a species known to possess glucose-6-phosphate dehydrogenase G6PD and urease Ure, grew rapidly on a nitrogen-deficient ND medium supplemented with NH4, urea, or any one of six amino acids. Comparable growth of related, but G6PD and Ure-, Pasteurella pestis was obtained only with L-glutamine or a combination NH4 plus glycine, L-threonine, or glyoxylate. Both species grew slowly on ND medium plus L-glutamate. Glycine alone supported slow growth of P. pseudotuberculosis and certain strains of P. pestis termed G and G mutants were selected from remaining strains of the latter species by use of ND agar plus glycine. ND agar plus NH4 was selective for a second class of P. pestis mutant termed N. Ure mutants of P. pestis were isolated on ND agar plus urea previously inoculated with N, G, cells similar inoculation with N, G-, organisms yielded only G mutants. Mutation of P. pestis to N, G, or Ure did not result in conversion to G6PD or to acquisition of other properties peculiar to P. pseudotuberculosis. The poor growth of wild-type P. pestis on ND medium plus NH4 is attributed to a deficiency of reduced nicotinamide adenine dinucleotide phosphate necessary for reductive amination of alpha-ketoglutarate. Author

Subject Categories:

  • Anatomy and Physiology
  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE