Accession Number:

AD0806448

Title:

BIOCHEMICAL CHANGES ASSOCIATED WITH CELL DIVISION IN USTILAGO SPHAEROGENA,

Descriptive Note:

Corporate Author:

ARMY MEDICAL RESEARCH LAB FORT KNOX KY

Personal Author(s):

Report Date:

1958-10-24

Pagination or Media Count:

11.0

Abstract:

Long and short cells uf U. sphaerogena, produced by different nitrogen sources, were analyzed for several cell constituents in an effort to correlate biochemical changes with differing rates of cell division. Various inhibiting agents might be expected to affect different reactions in a process as complex as cell division, and apparently several sites of action were observed in these experiments. Increased PNADNA ratios were observed in long cells grown on glycine, PNA, or aspartic acid plus ademine as nitrogen sources. Long cells grown on aspartic acid plus cysteine or aspartic plus succinate, and short cells showed lower ratios. All long cells, except those grown on aspartic acid plus succinate, which showed a greatly reduced total carbohydrate content conceivably responsible for reduced division, exhibited a low phospholipid content. A non-nucleic acid component, probably polyphosphates, of cellular hot TCA extracts tended to be high in long cells. These observations are discussed in light of the need to separate and study the metabolic systems and the specific reactions involved in the enormously complex process of cell division. Author

Subject Categories:

  • Biochemistry
  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE