THE INFLUENCE OF THE CHEMICAL COMPOSITION OF THE MEDIUM ON THE RESTORATION OF VIABILITY OF ULTRAVIOLET IRRADIATED ESCHERICHIA COLI, STRAIN B
ARMY MEDICAL RESEARCH LAB FORT KNOX KY
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The purpose of this study was to elucidate certain environmental influences which alter the viability of the ultraviolet irradiated bacterium, E. coli B. Suspensions of E. coli B when irradiated with ultraviolet light and subsequently incubated in the dark show a spontaneous rise in viable cell count. This increase in viability is demonstrable over a wide range of ultraviolet inactivation levels and is most pronounced with high doses of inactivating radiation. The increase in viability is dependent on the composition of the medium in which the cells are suspended. Potassium and phosphate ions as well as glucose were found to be essential for maximal dark reactivation.