Accession Number:

AD0806438

Title:

STEROID-PROTEIN INTERACTIONS. V. COMPARISON OF SPECTROPHOTOMETRIC AND EQUILIBRIUM-DIALYSIS PROCEDURES FOR DETERMINATION OF BINDING CONSTANTS,

Descriptive Note:

Corporate Author:

ARMY MEDICAL RESEARCH LAB FORT KNOX KY

Personal Author(s):

• Westphal, Ulrich
• Ashley, Billy D.
• Selden, George L.

Report Date:

1958-08-04

Pagination or Media Count:

13.0

Abstract:

An attempt was made to establish the validity of the spectrophotometric procedure for the determination of interaction between proteins and delta4-3-ketosteroids, this method was to be compared with the equilibrium-dialysis technique. The binding values obtained by the spectrophotometric method agree with those determined independently by equilibrium dialysis. The reduction of the extinction coefficient of an alpha, beta-unsaturated ketone by interaction with proteins provides an adequate basis for the determination of binding values. Author

Descriptors:

• (*STEROIDS
• BIOCHEMISTRY)
• (*PROTEINS
• BIOCHEMISTRY)
• SPECTROPHOTOMETERS
• EQUILIBRIUM(PHYSIOLOGY)
• ABSORPTION
• SERUM ALBUMIN
• HORMONES
• TESTOSTERONE
• PROGESTERONE
• CORTICOSTEROID AGENTS
• ULTRAVIOLET SPECTROSCOPY
• DIALYSIS

Subject Categories:

• Biochemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE