Accession Number:
AD0806438
Title:
STEROID-PROTEIN INTERACTIONS. V. COMPARISON OF SPECTROPHOTOMETRIC AND EQUILIBRIUM-DIALYSIS PROCEDURES FOR DETERMINATION OF BINDING CONSTANTS,
Descriptive Note:
Corporate Author:
ARMY MEDICAL RESEARCH LAB FORT KNOX KY
Personal Author(s):
Report Date:
1958-08-04
Pagination or Media Count:
13.0
Abstract:
An attempt was made to establish the validity of the spectrophotometric procedure for the determination of interaction between proteins and delta4-3-ketosteroids, this method was to be compared with the equilibrium-dialysis technique. The binding values obtained by the spectrophotometric method agree with those determined independently by equilibrium dialysis. The reduction of the extinction coefficient of an alpha, beta-unsaturated ketone by interaction with proteins provides an adequate basis for the determination of binding values. Author
Descriptors:
Subject Categories:
- Biochemistry