CELLULAR ADSORPTION OF PULMONARY SURFACE ACTIVE MATERIAL.
Semiannual progress rept.,
PENNSYLVANIA UNIV PHILADELPHIA DEPT OF PHYSIOLOGY
Pagination or Media Count:
An explanation was sought for the finding that lung washings, when centrifuged, yielded a sediment that exhibited strong surface-active properties, whereas the supernatant did not. Cells from lungs, when washed and shaken, lost this property, but it appeared in their diluent. The surfactant in this diluent could be readsorbed on washed cells obtained from lung washings, or on washed white cells obtained from the blood. It is concluded that 1. surfactant exists in a state in which it is adsorbed on the surface of cells obtained by washing the lungs, 2. surfactant can be desorbed by shaking and rinsing these cells from the lungs, 3. lung cells alone or their membranes and particles obtained by sonification lack such surface active property and 4. surfactant material desorbed from lung cells can be readsorbed onto washed lung cells or onto washed white blood cells by allowing the cells and surfactant to stand in contact overnight. Author
- Anatomy and Physiology