Allergenic and Antigenic Value of Tuberculoprotein Prepared from a Culture Filtrate by the Sodium Tungstate Method
FOREIGN TECHNOLOGY DIV WRIGHT-PATTERSONAFB OH
Pagination or Media Count:
The diagnostic value of allergenic and serologic reactions depends on the quality of the allergens and antigens involved. Thus, one of the more important elements in allergenic and serologic diagnosis of tuberculosis is to isolate and establish the standard complete antigens and allergens. In order to prepare the antigen, five strains of commercial microbacteria were seeded on synthetic media with citric acid. The cultivation in one thermostat lasted two months, at a temperature of 37 - 38 degrees. A dry, pure tuberculosis protein was produced by precipitation of the active initial filtrate by a 5 solution of sodium tungstate, followed by succeeding reprecipitation with a half-saturated solution of sodium sulphate. The residue obtained was dialized, poured into vials and exposed to lyophilic drying. The yield of tuberculosis protein was 2 - 3 times greater than when produced by another method.
- Medicine and Medical Research