Transovarial Transmission of California Encephalitis by Floodwater Mosquitoes,
WALTER REED ARMY INST OF RESEARCH WASHINGTON D C
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During arbovirus ecology studies on the eastern shore of Maryland, the Keystone strain of California encephalitis was repeatedly isolated from Aedes atlanticus mosquitoes. The field infection rate from this species remained approximately the same, both between years and during each year studies, even during periods when large numbers of adults were emerging. Further, virus was isolated from among the very first adult A. atlanticus collected and tested, thus suggesting that these mosquitoes had emerged infected. To demonstrate the suspected transovarial transmission of this virus, developmental stages of A. atlanticus were collected from the field. Larvae were identified and pooled for virus isolation in suckling mice. Pupae were allowed to emerge in the laboratory, then segregated by sex and pooled for virus isolation.