A Modified Histochemical Procedure for Measuring Succinic Dehydrogenase (SDH) in Corneal Epithelium.
Technical rept. Jun-Sep 72,
EDGEWOOD ARSENAL ABERDEEN PROVING GROUND MD
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Succinic dehydrogenase SDH, which is critical to maintaining the transparency of the cornea, is utilized in the aerobic metabolism of glucose via the tricarboxylic acid Krebs cycle. What happens to this enzyme when the cornea is deprived of normal concentrations of oxygen when contact lenses, masks, etc., are worn is not known. According to previous studies, SDH was either present in corneal epithelium in barely detectable amounts or the techniques used for measurement were not sensitive enough to detect all the SDH present. As a part of the study, the authors modified existing techniques of detecting SDH in corneal epithelium. The intensity of staining SDH was enhanced by modifying a medium containing tetrazolium to the point where it was sensitive enough for quantitative photometric measurements. The existing techniques were modified by adding a coenzyme, beta-diphosphopyridine nucleotide DPN, and increasing the amounts of specific substrate, sodium succinate. Author