Cryopreservation of Human Red Cells in Liquid Nitrogen with Hydroxyethyl Starch.
Technical annual rept. 1 Sep 73-31 Aug 74,
BLOOD RESEARCH INST BOSTON MASS
Pagination or Media Count:
Erythrocytes were frozen in plastic bags in liquid nitrogen with 14 wv hydroxyethylstarch HES. Both a small volume 25.0 ml. and a full unit system 405 ml. were employed. Small volume freezings were investigated to optimize container configuration, conditions, composition of media, and post-thawed stability of cells. Based on this experience a method for full unit freezing of packed erythrocytes was devised. The results of the ten most recent experiments with 25.0 ml. freezing gave post-thawed cell recoveries of 99.2 plus or minus 0.3. Stability for one hour in 0.15M NaCl at 22.0C. was 85.9 plus or minus 4.3. The cells gained 17 meq. of Na and lost 21meq. of K. ATP decreased 23 while 2,3 DPG was unchanged. In the large volume method using all the packed red cells in a unit after removal of platelet rich plasma and buffy coat a single bag was sandwiched between large perforated aluminum plates. The results of eight recent freezings and short term storage in liquid nitrogen vapor gave cell recoveries of 97.2 plus or minus 1.1, and stabilities in saline of 75.7 plus or minus 1.8. Relatively small losses of ATP and 2,3 DPG were observed. Modified author abstract
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