Significance of Leukocytes in Endotoxin Shock
OKLAHOMA UNIV HEALTH SCIENCES CENTER OKLAHOMA CITY
Pagination or Media Count:
Peripheral blood leukopenia and the sequestration of large numbers of polymorphonuclear leukocytes in pulmonary capillaries have been observed in experimental shock. It has been suggested that release of leukocytic lysosomal enzymes contributes to systemic hypotension and pulmonary capillary damage. This study was undertaken to evaluate the role of leukocytes in endotoxin shock. Six rhesus monkeys rendered leukopenic by total body irradiation were compared with six normal monkeys for two hrs after injection of E. coli endotoxin. Light and electron microscopy demonstrated sequestered polymorphonuclear leukocytes and platelets in pulmonary capillaries in the non-radiated group, but leukocytes were virtually absent in sections from lungs of the leukopenic animals. In spite of this difference, significant endothelial swelling and perivascular edema were demonstrable in both groups. No significant histologic abnormalities were noted in the three radiated leukopenic control animals who did not receive endotoxin. Leukopenia provided no protection from the hemodynamic effects or the histological damage in pulmonary capillaries observed after administration of endotoxin.
- Medicine and Medical Research