A Proposal to Study the Adrenal Cortex in Maintaining the Androgenic-Anabolic Status in Humans Under Normal and Stressful Experimental Conditions.
Progress rept., 1 Feb 73-31 Jan 74,
SOUTHWEST FOUNDATION FOR RESEARCH AND EDUCATION SAN ANTONIO TEX
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An antiserum for each of the three compounds, androstenedione, testosterone, and 11-Beta-hydroxyandrostendione, is now available permitting their measurement by highly sensitive, accurate radiommunoassay. However, the specificity of androstenedione antiserum is not sufficient to allow direct measurement with untreated plasma, thus preliminary chromatography is still required. The chromatography system has been scaled down so that samples obtained from one ml or less of plasma can be processed. There has been no loss in resolution or in sample recovery with the smaller column. Problems associated with the crucial first step extraction of the steroids from plasma have been successfully overcome. The laboratory personnel have gained considerable experience, expertise, and confidence in this period of methods development and are now prepared to perform analyses of many samples in a routine manner but with the scrupulous attention to detail that these assays demand. Author
- Stress Physiology