Accession Number:

AD0784632

Title:

Determination of Subpicogram Quantities of Drugs and Narcotics in Urine of Human Subjects.

Descriptive Note:

Interim annual rept. no. 1, 1 Mar 73-28 Feb 74,

Corporate Author:

STANFORD RESEARCH INST MENLO PARK CALIF

Report Date:

1974-03-01

Pagination or Media Count:

59.0

Abstract:

A feasibility study of the use of field ionization mass spectrometry for the analysis of drugs and narcotics in urine by isotope dilution was completed. Multilabeled methaqualone, 6-hydroxymethaqualone, morphine, and codeine were synthesized. The drugs and their isotopic carriers were extracted from urine on columns of Amberlite XAD-2 resin and were purified by one-dimensional thin-layer chromatography. The isotopic compositions of the extracted compounds were determined by mass spectrometry. Analyses of the methaqualone and 6-hydroxymethaqualone concentrations in a few urine samples obtained from human volunteers indicate that the sensitivity of the present technique is of the order of nanogramsml. More effective purification steps and the use of higher purity carrier compounds will permit the analysis of lower drug concentrations. Author

Subject Categories:

  • Biochemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE