Utilization of Gas Sparger for Submerged Culture Growth of L Cells and Manifold for Transfer Operations.
Technical rept. Feb 69-Jul 71,
EDGEWOOD ARSENAL ABERDEEN PROVING GROUND MD
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A gas sparger previously described for use in Bellco culture flasks has been tested in the laboratory to grow L-DR substrain mouse cells in a chemically defined medium. Adequate and continued propagation of L cells has been accomplished by using the criteria of doubling time, final population density, and recovery analysis following each 4-day passage. An inoculum of 5.24 x 1,00000.0 cells per milliliter 19.1 log base 2 units grew to a peak population level of 3.3 x 1,000,000.0 cells per milliliter 21.7 log base 2 units at the end of the log phase. A population doubling time of 25 hours was observed in the growth cycle of L-DR cells. In addition, the study showed that one ml of packed cell volume was equivalent to 5.46 x 1,000,000.0 cells. Extension of this value led to a determination of the productive output of the suspension gas culture system over a period of 32 days. The manifold-transfer apparatus performed according to design requirements for transfer operations. Author
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