The Microbial Degradation of Aromatic Petroleum Products.
Annual rept. no. 1, 25 Jun 73-24 Jun 74,
TEXAS UNIV AUSTIN DEPT OF MICROBIOLOGY
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It is the purpose of this contract to investigate the mechanisms used by soil bacteria to oxidize, ortho-, meta- and para-xylene. The progress report describes significant accomplishments including The isolation of a strain Pxy of Pseudomonas that will oxidize p- and m-xylene to carbon dioxide and water The isolation of a mutant strain of the above organism that accumulates p-toluic acid from p-xylene The isolation of a second mutant strain that accumulates 3- and 4-methylcatechol from m- and p-xylene The elucidation of the metabolic sequences used by Pseudomonas pxy to degrade p- and m-xylene to aliphatic products The isolation and identification of intermediates in the metabolism The isolation of the first bacterium reported to grow with o-xylene as the sole source of carbon and energy and, Identification of 3,4-dimethylcatechol as an intermediate in o-xylene metabolism.