Producing a Stable Toxin Clostridium Septicum from a Horsemeat Culture (Poluchenie Stavilnogo Toksina Clostridyum Septicum v Pitatelnoi Srede iz Konskogo Myaca)
ARMY FOREIGN SCIENCE AND TECHNOLOGY CENTER CHARLOTTESVILLE VA
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In preliminary experiments, the possibility of preparing the toxin Cl. septicum from a culture medium of horsemeat has been established, after which research was carried out on preparing the optimal culture medium. Different variants of nutritive media were prepared from acid hydrolysates of cooked and uncooked horsemeat a method of processing the meat before hydrolysis was worked out and the conditions for preparing the hydrolysates were changed. Hydrolysis was carried on in an open pot and at different pressures. The media that were prepared from hydrolysates differed in their proportions of the quantity of hydrolysates and the amount of beef and horsemeat broth that was added. In short, a technique was worked out for preparing a nutritive medium consisting of an acid hydrolysate and horsemeat broth. The influence of the length of growth, temperature, source of carbon food, and uterine culture on the toxigenesis of Cl. septicum, as well as the antigen structure and other properties of the toxin obtained was studied.