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DNA Precursors and the Absence of Thymidine Kinase in Yeast Mitochondria,
NATIONAL RESEARCH COUNCIL OF CANADA OTTAWA (ONTARIO) DIV OF BIOLOGICAL SCIENCES
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The absence of dTK in cell extracts of yeast and other microorganisms has been reported. However, the bromodeoxyuridine resistant mammalian cells which also lack the cytoplasmic enzyme, still contain a mitochondrion-specific dTK. The authors therefore asked whether yeast mitochondria contains a specific dTK and whether precursors other than dXTP can be used for the in vitro labelling of the m-DNA. The results show that yeast mitochondria do not contain any detectable levels of dTK and that among thymine derivatives tested only dTDP or dTTP could be used as effective precursors for the in vitro labelling of M-DNA.
APPROVED FOR PUBLIC RELEASE