Accession Number:

AD0780009

Title:

Some Effects of DBcAMP on Rabbit Bone Marrow Cells in Culture: Cell Clumping and Cholinesterase Production.

Descriptive Note:

Technical rept. Jul 72-Jul 73,

Corporate Author:

EDGEWOOD ARSENAL ABERDEEN PROVING GROUND MD

Personal Author(s):

• Garry,Vincent F. , Jr.
• Harris,Larrel W.
• Petrali,John P.

Report Date:

1974-04-01

Pagination or Media Count:

14.0

Abstract:

Bone marrow cells derived from phenylhydrazine-treated rabbits were grown in vitro for 24 hours. Marrow cultivated in the presence of .001 M dibutyryl 3,5-cyclic monophosphate DBcAMP formed large, dense cell clusters whereas those from control cultures formed smaller, more discrete cell clusters. Actinomycin D inhibited cluster formation. Erythroid cell maturation was also inhibited in the presence of DBcAMP. However, cholinesterase synthesis was enhanced. Most of the cells containing newly synthesized membrane-bound cholinesterase are erythroid. On the basis of the data obtained, it is suggested that the cyclic nucleotide may stimulate membrane protein synthesis. Author

Descriptors:

• *Bone marrow
• *Adenosine phosphates
• *Cholinesterase
• Biosynthesis
• Nucleotides
• Enzymes
• Cells(Biology)
• Proteins
• Membranes(Biology)
• Erythrocytes

Subject Categories:

• Biochemistry
• Anatomy and Physiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE