Studies on Addiction and Withdrawal in Cell Culture.
Annual rept. 1 Mar 73-28 Feb 74,
OKLAHOMA STATE UNIV STILLWATER DEPT OF BIOCHEMISTRY
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After 415 days 70 subcultures growth in the presence of slowly increased drug concentrations, KB cells with increased tolerance to amphetamine, benadryl, caffeine, codeine, and morphine have developed. No withdrawal symptoms have been observed upon the removal of amphetamine, or caffeine preliminary evidence for withdrawal symptons with benadryl and codeine have been obtained. Morphine withdrawal is also suggested. During this continuous culturing in the presence of drugs altered cellular morphology has been observed with the following drugs aspirin, chloropromazine, codeine, morphine, and valium. Some of these changes persist after drug removal suggesting that a different cell population has been selected. Growth of fresh cells in the presence of LD50 or morphine reduces cell division, increases vacuolation, and markedly decreases the extent of membrane movement. These effects are all readily reversible upon removal of morphine from the KB culture. Similar results have been obtained with neuroblastoma cells. Author