Local Graft Versus Host Reaction Suppressed by Immunoblocking Antibody Secreted by Antibody-Forming Cells Transferred with the Graft
ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD
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The purpose of the study was to test the hypothesis that the reduction in graft versus host GVH activity following donor sensitization is due to active enhancement and, furthermore, that the immunosuppression is mediated by antibody secreted by cells transferred with the graft. To this end, the popliteal lymph node PLN assay was used to measure the GVH reactivity of parental rat spleen cells injected into adult F1 hybrid recipients. Immunization of the donor with an intravenous injection of hybrid spleen cells resulted in a reduction in GVH reactivity. The magnitude of the immunosuppression depended upon the interval between immunization and assay and was associated with the level of cytotoxic antibody in the serum of the donor. Using velocity sedimentation to separate immunized spleen cells, the authors were able to show that cells from the fraction enriched in antibody-forming cells AFC can inhibit the GVH reactivity of normal spleen cells.
- Medicine and Medical Research