Accession Number:

AD0775034

Title:

The Effects of Maleylation on the Properties of Concanavalin A,

Descriptive Note:

Corporate Author:

NATIONAL RESEARCH COUNCIL OF CANADA OTTAWA (ONTARIO) DIV OF BIOLOGICAL SCIENCES

Personal Author(s):

Report Date:

1973-06-19

Pagination or Media Count:

7.0

Abstract:

The major product resulting from treatment of concanavalin A with maleic anhydride is a stable, dimeric species, devoid of subunits with broken peptide chains. For complete dissociation of the concanavalin A tetramer, at least eight maleyl groups per subunit were required. The binding site in the fully modified protein was shown to be essentially unchanged, by equilibrium dialysis experiments with para-nitro-phenyl-alpha-D-glucoside. Precipitation reactions with dextran were altered, and would only proceed in the presence of high salt concentration at acid pH. Affinity chromatography on Sephadex G-75 showed that the maleylated dimer behaved in a similar manner to the dimeric form of concanavalin A. Author

Subject Categories:

  • Biochemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE