Separation of Dialyzable Peptide Allergens from Tuberculous and Tularemia Bacteria,
FOREIGN TECHNOLOGY DIV WRIGHT-PATTERSON AFB OHIO
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The possibility of extracting allergens directly from a bacterial mass is of major practical interest and attracts the attention of many researchers. A tuberculous allergen was separated from tuberculosis mycobacteria, brucellosis allergen from brucelli, and dysentery diagnosticum from dysentery brucelli. Other methods of extracting allergens from bacteria are also known extraction by an alkali, extraction by buffer solutions and by acids. The separation of allergens directly from a bacterial mass makes it possible to obtain active and high-quality allergens from production wastes at biological manufacturing plants, and to extract in quantities substantially exceeding the quantity of allergens usually obtained from cultural filtrates. Another important fact is that the allergens contain substantially fewer foreign impurities of polysaccharides and nucleic acids. The OKada method used to separate allergens of a peptide nature directly from a bacterial mass of production strains of tuberculosis mycobacteria strain DtSt and from tularemia bacteria the vaccine strain 15-V 2S is described.