Study of the Protein Components of Venezuelan Equine Encephalomyelitis with Use of Electrophoresis and Isoelectric Focussing
ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD
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The presence of three protein components with molecular weights of 59,000, 34,000 and 18,000 daltons was determined from mobility in polyacrylamide gel containing sodium dodecysulfate. The method of isoelectric focussing in two modifications column focussing and focussing on the basis of disk polyacrylamide gel was used to demonstrate also the presence of three protein- containing components in low-acid, neutral and low alkaline zones of pH gradient both by ultraviolet adsorption and by radioactive label.