Yeast Phenylalanyl Transfer Ribonucleic Acid Synthetase: Purification and Complex Formation with Phenylalanine Transfer Ribonucleic Acid.
Technical rept. Nov 72-May 73,
EDGEWOOD ARSENAL ABERDEEN PROVING GROUND MD
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It has been found that a homogeneous preparation of phenylalanyl aminoacyl- tRNA synthetase phe-synthetase from yeast can be obtained if phenylmethylsulfonyl fluoride is used in all buffers and if phosphocellulose chromatography is employed as a terminal step in the purification. Molecular weight was determined, and complexes between phe-synthetase and tRNAPhe were observed in the analytical ultracentrifuge. Modified author abstract