Accession Number:

AD0770376

Title:

Yeast Phenylalanyl Transfer Ribonucleic Acid Synthetase: Purification and Complex Formation with Phenylalanine Transfer Ribonucleic Acid.

Descriptive Note:

Technical rept. Nov 72-May 73,

Corporate Author:

EDGEWOOD ARSENAL ABERDEEN PROVING GROUND MD

Report Date:

1973-11-01

Pagination or Media Count:

25.0

Abstract:

It has been found that a homogeneous preparation of phenylalanyl aminoacyl- tRNA synthetase phe-synthetase from yeast can be obtained if phenylmethylsulfonyl fluoride is used in all buffers and if phosphocellulose chromatography is employed as a terminal step in the purification. Molecular weight was determined, and complexes between phe-synthetase and tRNAPhe were observed in the analytical ultracentrifuge. Modified author abstract

Subject Categories:

  • Biochemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE