Activity of Some Glycolysis Enzymes in the Liquid Phase of Tissue Cultures Infected with Venezuelan Equine Encephalomyelitis Virus,
ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD
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The reproduction of Venezuelan equine encephalitis virus in cultures of chick and human embryo fibroblasts is accompanied by changes in the activity of some enzymes of carbohydrate metabolism glucosophosphatisomerase, aldolase, and lactatedehydrogenase. The enzymatic changes in the cells were determined by the changes in enzymatic activity in the tissue culture fluids. The experiments performed demonstrated that the activity of glucosophosphatisomerase increases so early as 4 hours after infection of fibroblasts, before cytopathic changes develop in the cells. Lactatedehydrogenase activity also exhibited a statistically significant increase before the appearance of cytopathic changes. Unlike the activity of these two enzymes, the aldolase activity of the medium did not change in advance of cell sheet degeneration and did not increase until after the death of the cells.