Accession Number:

AD0770292

Title:

Ultrastructural Pathogenesis of Lesions Produced by Exposure to Oxygen Difluoride with Correlative Light Microscopy

Descriptive Note:

Corporate Author:

NATIONAL AERONAUTICS AND SPACE ADMINISTRATION MOFFETT FIELD CA AMES RESEARCH CENTER

Personal Author(s):

• Harrison, Gladys
• Mackenzie, William

Report Date:

1973-07-01

Pagination or Media Count:

56.0

Abstract:

The lungs of rats exposed to OF2 were examined by light and electron microscopy. The exposures were for 30 to 60 minutes to an average of 4.5 ppm OF2, the minimal lethal dose. Animals were sacrificed after 30 group 1 and 60 minutes group 2 exposure and 1 group 3 and 2 group 4 hours following 60 minutes exposure. Mild gross changes were observed in groups 3 and 4, but no light microscopic lesions were found. Alterations were noted in all four groups using electron microscopy. These were mostly indicative of fluid change and consisted of blebbing of the endothelial and epithelial layers of the alveolocapillary wall and rarification of the cytoplasm of these cells. The lamellar bodies of the Type II cells showed an increasing and consistent loss of matrix structure and density. These fine structural changes increased in quantity and severity as time of exposure or post-exposure period increased.

Descriptors:

• *TOXICITY
• *LUNG
• *FLUORIDES
• TISSUES(BIOLOGY)
• HISTOLOGICAL TECHNIQUES
• PATHOLOGY
• MICROSCOPY
• DOSAGE

Subject Categories:

• Toxicology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE