Accession Number:

AD0768951

Title:

Orale Immunisierung mit Nichtvermehrungsfaehigen Mikroorganismen oder ihren Antigenen. 12. Mitteilung: Immunogenitaet Verschieden Inaktivierter S. Typhi Murium-Vollantigene im Maeuseschutztest (Oral Immunization with Non-Living Microorganisms or Their Antigens. 12),

Descriptive Note:

Corporate Author:

ROBERT KOCH-INSTITUT BERLIN (WEST GERMANY)

Report Date:

1970-05-21

Pagination or Media Count:

14.0

Abstract:

White mice were on ten subsequent days orally immunised by an oesophageal sound. Seven to fourteen days following the last oral vaccination all of the mice were infected orally with virulent S. typhi murium bacteria. The infective dose was adjusted to LD50. The vaccine consisted of S. typhi murium bacteria inactivated by different physical or chemical methods. The protein-denaturating inactivation methods DMSO and HBO-uv-rays destroy the immunogenicity. Vaccines obtained by application of acetone inactivation, formaldehyde-treatment, DNA-absorbed uv-rays and low temperatures yield medium protection values. The immunogenicity of the vaccines is improved by increasing the inactivation temperature and decreasing the period of treatment. The most effective vaccine was obtained by heating to 100C for three minutes. Modified author abstract

Subject Categories:

  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE