A Study of the Complexing of Viral RNA with Cell Proteins
ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD
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Information of some physico-chemical properties of chick fibroblast proteins forming in vitro complexes with Venezuelan equine encephalomyelitis virus RNA has been obtained. Soluble cell proteins which are not sedimented by centrifugation at 105,000g have been shown to form complexes with viral RNA. These proteins adsorb solidly on DEAE-cellulose at pH 6.8 and are eluted from it only with 0.3 M NaCl. The sedimentation analysis revealed three classes of proteins according to the sedimentation constant which form complexes with RNA. It has been demonstrated that the process of complexing of viral RNA with cell proteins occurs most effectively in solutions of low and moderate ion strength. The fact that RNA complexing with protein is inhibited by high concentrations of salt suggests participation in it of electrostatic forces. The relationship between the sedimentation rate of the resulting ribonucleoprotein complexes and the amount of protein complexing with RNA has been established.