Accession Number:

AD0768737

Title:

Studies of the Mammalian Brain Function in vitro.

Descriptive Note:

Annual rept. no. 7, Dec 70-Dec 72,

Corporate Author:

KOBE UNIV (JAPAN) DEPT OF PHYSIOLOGY (2ND)

Personal Author(s):

Report Date:

1972-12-01

Pagination or Media Count:

24.0

Abstract:

Effects of long term 7 14 and 5 34 years storage of the cat in frozen state at -20C in vitro after employing a glycerol perfusion technique were investigated from modes of neuroelectrical activity and histological evidences. The following conclusions were obtained Glycerol, even if administered via vascular systems, showed cryoprotective effects on the brain tissues as was the case of single cells it was possible to preserve viability of nervous tissues against many years storage in frozen state reperfusion for revival brought about enlargement of micro-clefts in tissues which might have been produced by thawing of long term frozen tissues. This may be a cause of bleeding and activity defect in the revived brain integrated neuroelectrical activity such as brain waves and evoked potentials may be affected significantly by dissolution of functions resulted from disruption in tissues individual nerve cell activity, on the contrary, may be preserved well essentially or augmented in activity by degenerative processes. Author

Subject Categories:

  • Anatomy and Physiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE