Studies of the Mammalian Brain Function in vitro.
Annual rept. no. 7, Dec 70-Dec 72,
KOBE UNIV (JAPAN) DEPT OF PHYSIOLOGY (2ND)
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Effects of long term 7 14 and 5 34 years storage of the cat in frozen state at -20C in vitro after employing a glycerol perfusion technique were investigated from modes of neuroelectrical activity and histological evidences. The following conclusions were obtained Glycerol, even if administered via vascular systems, showed cryoprotective effects on the brain tissues as was the case of single cells it was possible to preserve viability of nervous tissues against many years storage in frozen state reperfusion for revival brought about enlargement of micro-clefts in tissues which might have been produced by thawing of long term frozen tissues. This may be a cause of bleeding and activity defect in the revived brain integrated neuroelectrical activity such as brain waves and evoked potentials may be affected significantly by dissolution of functions resulted from disruption in tissues individual nerve cell activity, on the contrary, may be preserved well essentially or augmented in activity by degenerative processes. Author
- Anatomy and Physiology