Accession Number:

AD0768642

Title:

The Influence of Gamma-radiation on the Structure and Biological Activity of Oxytocin, a Cyclic Oligopeptide,

Descriptive Note:

Corporate Author:

NATIONAL RESEARCH COUNCIL OF CANADA OTTAWA (ONTARIO) DIV OF BIOLOGICAL SCIENCES

Personal Author(s):

Report Date:

1972-11-17

Pagination or Media Count:

7.0

Abstract:

During irradiation in aqueous solution under various conditions, the biological activity of oxytocin was lost exponentially, and the following D37 doses were found aerated, 270 krads deaerated, 230 krads N2O saturated, 170 krads deaerated butanol, 440 krads. Thus, the hydroxyl radical appeared to be the principal inactivating species. Hydrolysis and analysis of irradiated hormone showed that cystine and tyrosine were the main residues affected. Irradiated oxytocin was resolved into three fractions by gel filtration of Sephadex G-15 The first fraction, which was inactive, predominated in deaerated solution and appeared to consist of dimer or polymer. The third fraction was the major one from aerated and N2O-saturated solutions and had a potency close to that of the original hormone. Author

Subject Categories:

  • Biochemistry
  • Radiobiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE