Characteristics of Virion RNA of Venezuelan Equine Encephalomyelitis Virus
ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD
Pagination or Media Count:
Certain physico-chemical parameters of infectious RNA of the Venezuelan equine encephalomyelitis virus were determined. It was established that virion RNA sediments in sucrose gradient with sedimentation coefficient of 38-40 S. In fractionation of RNA in cesium sulfate density gradient, the RNA was found in one zone of 1.66 gcc density. Electrophoresis of virion 40 S RNA in 3.5 agarose-polyacrylamid gel supplied additional information on RNA preparations, making it possible to determine the molecular weight of RNA, which, in several different experiments, varied within the limits of 4,000,000 and 4,300,000 daltons.