Studies on Mammalian and Human Pyruvate and alpha-Ketoglutarate Dehydrogenation Complexes.
Annual rept. no. 12, 15 Mar 72-14 Mar 73,
NAGASAKI UNIV (JAPAN) ATOMIC DISEASE INST
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Enzyme systems that catalyze a coenzyme A- and nicotinamide adenine dinucleotide-linked oxidative decarboxylation of pyruvate and 2-oxoglutarate in mammals have been isolated from the Keilin-Hartree preparation of the pig heart muscle as soluble multienzyme complexes with molecular weights in the millions. These multienzyme complexes have distinctive structures and catalyze a coordinated sequence of reaction. The aims of this research are to resolve these complexes into their essential enzymes, to characterize the latter and to determine the composition, macromolecular structure and regulatory mechanism of these two multienzyme complexes. Modified author abstract