The Vacuole as a Sugar Compartment in 'Saccharomyces cerevisiae'.
ARMY MEDICAL RESEARCH LAB FORT KNOX KY
Pagination or Media Count:
Sorbose influx into yeast previously incubated in 100 mM glucose solutions resulted in more complete equilibration with external sorbose than occurred in yeast incubated in water. Efflux from the glucose-incubated organisms occurred primarily at an initial rapid rate rather than at two different rates, as occurred from H2O-incubated yeast and as occurs from fresh yeast. These differences were apparent after 6 hr of incubation and indicated the lack of a normally occurring intracelluar sorbose compartment in the glucose-incubated organisms. Concurrent with the changes in uptake and efflux, the cell vacuole disappeared, evidence that it is the sorbose compartment. A rapid loss of 260 micrometers absorbing materials indicated that glucose incubation caused an immediate loss of vacuolar contents, apparently resulting ultimately in elimination of the vacuole and an increase in the dry weight per volume of the organisms. Many lipid bodies formed in glucose-incubated yeast, evidently because of the available glucose. Author