Universal Virus Adsoprtion on Inert Particles Utilizing the Coated Latex Adsorption Method (CLAM).
Annual rept. 31 Jul 72-30 Jun 73,
SOUTH DAKOTA STATE UNIV BROOKINGS DEPT OF MICROBIOLOGY
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A rapid, easy, universal process CLAM was devised for detecting and identifying human pathogenic virues and specific antibodies. Quantititive adsorption and detection of virus by radio-immuno-assay RIA, Coulter counter system, and fluorochrome tagged enzyme was achieved using latex particles and CLAM process. Modular instrumented packages were assembled and tested. A mini-computer and computer programs specifically designed for rapid quantitation of viral particles and their antibody molecules were used to compute the results within seconds. Data and conclusions for theoretical, experimental, and instrumental proof of the application of CLAM process for virus detection are presented in detail in the report. This research showed that it is possible to develop automated instruments to detect and count viruses within minutes. Author