Investigation of the Prolonged Recovery Time Following Botulinum Intoxication. Efficacy of Intraperitoneal versus Intravenous Injection of Botulinum Antitoxin.
Final rept. 10 Jul 1972-10 Jul 1973
WOODARD RESEARCH CORP HERNDON VA
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In order to study prolonged recovery times, mice were injected via the intramuscular route with sublethal concentrations of botulinum type A toxin. Proteolytic enzyme solutions were injected into the test site to determine if the removal of bound proteinacious toxin from neuromuscular junctions could influence paralytic recovery times. Trypsin and protease at 1 mg concentrations appeared to markedly influence recovery whereas chymotrypsin, papain, ficin, pepsin and CaCl2 did not significantly affect decrease of paralysis. The efficacy of intraperitoneal versus intravenous injection of botulinum antitoxin was studied by initiating a state of paralysis with resulting death of groups of mice and prophylactically administering specific antitoxin by either the intravenous or intraperitoneal routes. There appeared to be no effect of route of antitoxin administration on protective action of antisera therapy.