Increased Translation in Liver Involves a Supernatant Factor Following Laser Irradiation,
YORK UNIV DOWNSVIEW (ONTARIO) DEPT OF BIOLOGY
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Because of the increasing use of CO2 lasers and the importance of the liver to life processes, the authors have irradiated the abdomen of rats with CO2 laser 10 600 A and subsequently examined protein synthesis in the liver. The incorporation of 14C-phenylalanyl-tRNA into protein was increased in cell-free preparations of the liver. The results were different from those reported for regenerating liver since the increased incorporation was observed in ribosome preparations as well as in microsome preparations. Moreover, the increase occurred with endogenous mRNA or with synthetic mRNApolyU and was chiefly due to a factor in the supernatant fraction of the liver. An increased binding of aminoacyl-tRNA, which depends on the activity of elongation factor 1EF 1 appears to be responsible for the increased synthesis of peptide.